                         SEQUENCE LISTING

<110>  Instytut Biochemii i Biofizyki
 
<120>  DNA vaccine against H5N1 influenza virus, modified nucleotide 
       sequence and use of the modified nucleotide sequence in vaccine 
       manufacturing

<130>  25425/PCT

<140>  PCT/PL2015/000
<141>  2015-06-17

<150>  PL411230
<151>  2015-02-10

<160>  2     

<170>  PatentIn version 3.5

<210>  1
<211>  1686
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  a nucleotide sequence coding H5 HA (GK) with deletion (?RRRKKR) 
       of the hemagglutinine cleavage site into HA1 and HA2 subunits

<400>  1
atggagaaga tcgtgctgct gttcgccatc gtgagcctgg tcaagtccga ccagatctgc       60

atcggctacc acgccaacaa cagcaccgag caggtggaca ccatcatgga gaagaacgtg      120

accgtgaccc acgcccagga catcctggag aagacccaca acggcaagct gtgcgacctg      180

gacggcgtga agcccctgat cctgcgcgac tgcagcgtgg ccggctggct gctgggcaac      240

ccgatgtgcg acgagttcct gaacgtgccc gagtggagct acatcgtgga gaagatcaac      300

cccgccaacg acctgtgcta ccccggcaac ttcaacgact acgaggagct gaagcacctg      360

ctgagccgga tcaaccactt cgagaagatc cagatcatcc ccaagagcag ctggtccgac      420

cacgaggcca gcagcggcgt gagcagcgcc tgcccctacc agggccgcag ctccttcttc      480

cggaacgtgg tgtggctgat caagaaggac aacgcctacc ccaccatcaa gcgcagctac      540

aacaacacca accaggagga cctgctggtg ctgtggggca tccaccaccc caacgacgcc      600

gccgagcaga cccgcctgta ccagaacccc accacctaca tcagcgtggg caccagcacc      660

ctgaaccagc ggctggtgcc caagatcgcc acccgcagca aggtgaacgg ccagtccggc      720

cgcatggagt tcttctggac catcctgaag cccaacgacg ccatcaactt cgagtccaac      780

ggcaacttca tcgcccccga gaacgcctac aagatcgtga agaagggcga cagcaccatc      840

atgaagagcg agctggagta cggcaactgc aacaccaagt gccagacccc catcggcgcc      900

atcaactcca gcatgccctt ccacaacatc caccccctca ccatcggcga gtgccccaag      960

tacgtgaaga gcaaccgcct ggtgctggcc accggcctgc ggaactcccc ccagggcgag     1020

ggcctgttcg gcgccatcgc cggcttcatc gagggcggct ggcagggcat ggtggacggc     1080

tggtacggct accaccacag caacgagcag ggcagcggct acgccgccga caaggagagc     1140

acccagaagg ccatcgacgg cgtgaccaac aaggtgaaca gcatcatcaa caagatgaac     1200

acccagttcg aggccgtggg ccgcgagttc aacaacctgg agcggcgcat cgagaacctg     1260

aacaagaaga tggaggacgg cttcctggac gtgtggacct acaacgccga gctgctggtg     1320

ctcatggaga acgagcgcac cctggacttc cacgacagca acgtgaagaa cctgtacgac     1380

aaggtgcgcc tgcagctgcg ggacaacgcc aaggagctcg gtaacggctg cttcgagttc     1440

taccaccggt gcgacaacga gtgcatggag agcgtgcgca acggcaccta cgactacccc     1500

cagtactccg aggaggcccg cctgaagcgg gaggagatca gcggcgtgaa gctggagagc     1560

atcggcacct accagatcct gagcatctac tccaccgtgg cgagcagcct ggccctggcc     1620

atcatggtgg ccggcctgtc cctgtggatg tgcagcaacg gctccctgca gtgccgcatc     1680

tgcatc                                                                1686


<210>  2
<211>  1689
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  a nucleotide sequence coding H5 HA (K3) with deletion (?RRRKKR) 
       of the hemagglutinine cleavage site into HA1 and HA2 subunits

<400>  2
atggaaaaga ttgtgctgct gtttgctatc gtgtccctgg tgaaaagcga ccagatttgt       60

atcgggtatc atgccaataa ctcaaccgaa caggtggata ctattatgga gaaaaacgtg      120

actgtgaccc acgcccagga catcctggag aagacccata acggcaaact gtgcgatctg      180

gacggagtga agcccctgat cctgcgcgat tgcagcgtgg ctggctggct gctgggaaac      240

cctatgtgcg acgagttcct gaatgtgcca gaatggtcct acatcgtgga gaaaattaac      300

ccagcaaatg atctgtgcta ccccggcaac ttcaatgact atgaggaact gaagcacctg      360

ctgtctagga tcaaccattt cgaaaagatc cagatcatcc ctaagagctc ctggagcgat      420

cacgaggctt cttcaggcgt gagtagcgca tgtccatacc agggacgctc ctctttcttt      480

cggaacgtgg tgtggctgat taagaaagac aatgcttacc caactatcaa acgcagctat      540

aacaatacca accaggaaga tctgctggtg ctgtggggaa tccaccatcc caacgacgcc      600

gctgagcaga cacggctgta ccagaatcct accacatata ttagtgtggg gacaagcact      660

ctgaaccaga gactggtgcc caagatcgca acaaggagca aagtgaatgg ccagtccgga      720

agaatggagt tcttttggac tatcctgaag ccaaacgatg ccattaattt cgaatctaac      780

ggcaacttca tcgcacccga gaacgcctac aagattgtga agaaaggaga ctccactatc      840

atgaaatctg agctggaata tgggaactgc aataccaagt gtcagacacc tatcggtgca      900

attaactcaa gtatgccctt tcacaatatc catcctctga ccattgggga gtgccccaag      960

tacgtgaaaa gtaaccgcct ggtgctggcc acaggtctgc ggaatagccc tcagggggaa     1020

ggtctgttcg gggctatcgc aggttttatt gagggcggat ggcagggaat ggtggatggg     1080

tggtacggtt atcaccattc aaacgaacag ggcagtggat acgcagccga taaggagtca     1140

acacagaaag ccattgacgg agtgactaac aaggtgaact ccatcattaa caaaatgaac     1200

acccagttcg aggctgtggg gagagagttc aacaatctgg agagaaggat cgaaaacctg     1260

aataagaaaa tggaagatgg cttcctggac gtgtggactt acaacgctga gctgctggtg     1320

ctgatggaga atgaaaggac cctggatttt cacgacagca acgtgaagaa tctgtatgat     1380

aaagtgagac tgcagctgag ggacaacgca aaggaactgg ggaatggttg tttcgagttt     1440

taccatagat gcgataacga gtgtatggaa tccgtgagga atggcacata cgactatcca     1500

cagtattctg aggaagcccg cctgaagcgg gaggaaattt ctggggtgaa actggagtca     1560

atcggtacct accagatcct gtctatctac tcaacagtgg ctagctccct ggccctggct     1620

atcatggtgg ctggcctgag cctgtggatg tgctctaacg gtagcctgca gtgtaggatc     1680

tgtatttga                                                             1689


